General description

Recombinant, human p34^Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, Cdk1 is inactive and requires association with cyclin B and phosphorylation of Thr¹⁶¹ to form an active complex. MW: 34000(p34^Cdk1) and 55000(cyclinB).

Recombinant, human p34^Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, p34^cdc2 is inactive and requires association with cyclin B to form an active complex.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.

Packaging

200 u in Plastic ampoule

Physical form

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ^® 35 Detergent, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)